TABLE III.

Brood Size and Motility of the Mutated Worms

	Brood Size (average ± SD, n = 5)			Motility (beats/30 sec: average ± SD, n ± 10)
	15°C	20°C	25°C	15°C	20°C	25°C
WT	327 ± 17	249 ± 16	166 ± 34	112 ± 1.7	104 ± 4.9	94.5 ± 4.5
pfn-2(null)	298 ± 20	270 ± 49	155 ± 23	115 ± 6.8	111 ± 8.0	89.1 ± 7.2
pfn-3(null)	226 ± 14	285 ± 39	144 ± 28	110 ± 5.6	90 ± 3.8	84.1 ± 5.4
WT; control RNAi	275 ± 58	301 ± 22	166 ± 34	100 ± 6.5	118 ± 4.9	89.3 ± 4.6
WT; pfn-2(RNAi)	283 ± 21	326 ± 23	152 ± 18	100 ± 3.5	103 ± 3.6	88.6 ± 5.3
pfn-3(null); control RNAi	268 ± 36	254 ± 35	125 ± 14	88.0 ± 7.6	96.3 ± 5.3	87.1 ± 4.4
pfn-3(null); pfn-2(RNAi)	276 ± 33	285 ± 23	117 ± 5.4	92.0 ± 5.0	106 ± 7.4	88.8 ± 6.6

These measurments were performed at the indicated temperatures. WT: wild-type C. elegans, pfn-2(null): pfn-2 null mutant, pfn-3(null): pfn-3 null mutant, WT; control RNAi: wild-type worm treated with the RNAi vector with no insert; WT; pfn-2(RNAi): RNAi of pfn-2, pfn-3(null); control RNAi: pfn-3 null mutant treated with the RNAi vector with no insert; and pfn-3(null); pfn-2(RNAi): pfn-3 null mutant with RNAi of pfn-2.