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. Author manuscript; available in PMC: 2009 May 15.
Published in final edited form as: J Immunol. 2008 May 15;180(10):7039–7046. doi: 10.4049/jimmunol.180.10.7039

Table III.

mTg1677 is pathogenic in A+E− and A+E+ mice

Thyroiditis No. of Mice with% Thyroid Involvement
Strain Treatmenta Immunizationb No. Activated Cells Transferredc 0 >0–10 >10–20 >20–40 % Positive/Total
A+E− - mTg1677 - 8 - - - 0
anti-CD25 (donors)c mTg1677 - 16 2 1 - 16
irradiate (recipients)c - 6–7 × 107 6 1 2 1 40
A+E+ - mTg1677 - 6 4 - - 40
anti-CD25 mTg1677 - 3 2 - 1 50
a

Mice were untreated or depleted of CD25+ Tregs by i.v. injection of 1 mg CD25 mAb (PC61) 10 and 14 days before immunization.

b

Mice were immunized with 100 μg mTg1677 emulsified in CFA on days 0 and 7, and thyroids were obtained on day 28 or 35.

c

SC of Treg-depleted, mTg1677-immunized A+E− donor mice were incubated for 3 days with 5–10 μg/ml mTg1677 before injection into syngeneic, irradiated (500 rads) recipients.