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. 2008 Oct 23;105(43):16484–16489. doi: 10.1073/pnas.0806574105

Fig. 1.

Fig. 1.

VHL status affects sensitivity to shRNA-mediated kinase inhibition. (A) Raw alamarBlue fluorescence values for 786-O cells infected with lentiviruses encoding kinase shRNAs. Red and black bars depict values for 786-O cells infected with a retrovirus encoding HA-pVHL or with the empty vector, respectively. The x-axis indicates rank order of shRNAs based on corresponding alamarBlue values after infection of the empty vector cells. See Table S2 for gene names. (B and C). Difference in percentage loss of viability (Δ %LOV), after normalization (see text) for 786-O cells (B) and RCC4 cells (C) infected with lentiviruses encoding kinase shRNAs. shRNAs that preferentially inhibit VHL−/− cells appear on the right, and the pVHL-restored cells to the left. See Tables S4 and S5 for gene names. Lentiviruses that scored positively in 786-O cells (Δ %LOV > 30% in B) are indicated with yellow bars in C.