Fig. 2.

Kinase shRNAs that preferentially inhibit VHL−/− renal carcinoma cells. (A) Heat map depicting the effects of the 100 shRNA vectors on the two cell-line pairs after normalization to the GFP cDNA and scrambled shRNA controls (see text). Hierarchical clustering analysis using the Manhattan clustering method was done using the TM4 program (35). Experiments or shRNAs with relatively similar patterns are clustered together. shRNAs causing > 50% loss of viability are indicated in red, and those with < 50% loss of viability are in green. (B) List of shRNA vectors (kinase name followed by TRC collection shRNA number) that scored positively (see text for criteria) when tested in 786-O and RCC4 isogenic cell-line pairs that did (VHL) or did not (vector) produce wild-type pVHL. (C) Photomicrographs (100 × magnification) of crystal violet-stained 786-O cells 5 days after infection with lentiviruses encoding the indicated shRNAs. scr = scrambled shRNA vector.