Table 2.

Analysis of missense-suppressed thymidylate synthase in ΔthyA background

Reporter allele	Added aaRS	Active ThyA[146C], pmol/mg S100	Total ThyA[146C] + ThyA[146P], pmol/mg S100	Suppression, %*
thyA		800 ± 80	800	100
ΔthyA		0	0
thyA:146CCAC→P		3.8 ± 1	640	0.6
thyA:146CCAC→P	GLproS	137 ± 13	685	20
thyA:146CCAC→P	ECproS	24.7 ± 1.6	561	4.4
thyA:146CCAC→P	BBproS	8.7 ± 1	483	1.8

*Suppression % represents the ratio between the active and total reporter proteins.