Fig. 1.

No J-protein other than Sis1 is required for maintenance of [RNQ⁺] or [PSI⁺]. [RNQ⁺] (A) or [PSI⁺] (B) cells lacking individual J-proteins were passaged for 3 weeks on rich media before analysis. (A) For analysis of [RNQ⁺], cell lysates were prepared, resolved by SDD-AGE and subjected to immunoblotting using Rnq1-specific antibodies. Dotted lines indicate lanes from different parts of the same gel. Non-specific bands appearing in all lanes at the bottom of the gel have been cropped for clarity. (B) For [PSI⁺], cells were streaked onto rich medium and grown at 22 °C for color development. Control WT [PSI⁺] and [psi⁻] cells were included for comparison. Analysis of extracts from these strains by SDD-AGE was performed (Fig. S1). Additionally, as expected, all [PSI⁺] strains maintained the ability to grow in the absence of adenine (data not shown).