Fig. 2.

The ClpX-mediated degradation of CpdR is dependent on its phosphorylation state and on DivK. (A) WT cells harboring the plasmid pP_xylX-clpX* (14) were grown in M2G medium in the absence of xylose. Culture samples were incubated for 4 h either in the absence (solid line) or in the presence of xylose to allow clpX* induction (dashed line). (B) Pulse–chase experiments showing the half-lives of CpdR (black lines) and CpdR_D51A (gray lines) in the presence (solid lines) or absence (dashed lines) of DivK. Each strain was incubated in the presence of xylose to induce cpdR or cpdR_D51A and in the presence or absence of vanillate (presence or absence of DivK, respectively). R² is the regression coefficient.