Fig. 5.
The presence of the unphosphorylatable CpdRD51A bypasses the requirement of DivK for cell survival. (A) Four indicated strains were grown in PYE media in the presence of vanillate (divK expression) and xylose (cpdR or cpdRD51A expression) to an O.D.660 of 0.6. The cultures were serially diluted, and 5 μl of each sample was spotted onto PYE plates containing no vanillate but xylose (Left), and no vanillate but glucose (Right). (B) Cells from the strains ΔcpdR ΔdivK PvanA-divK pPxylX-cpdR (i–v) and ΔcpdR ΔdivK PvanA-divK pPxylX-cpdRD51A (vi–x) were grown in M2G media in the presence of vanillate (presence of DivK) and xylose (presence of CpdR or CpdRD51A). Culture samples were washed and incubated in M2G media in the presence or absence of vanillate or xylose before microscopic observation. All cultures were kept in exponential phase growth for the entire experiment.