Table 2.

Summary of the molecular markers in IGL protein sequences

Gene	Position	Markers	κ	λ	σ
IGLV	7	Gap S/T	− (100)+ (97)	+ (100)− (97)	− (100)+ (100)
	41a	Gap	+ (100)	+ (100)	− (100)
	46 a–b	Gap	+ (100)	+ (100)	− (100)
	53	F/Y	+ (100)	− (100)	+ (100)
	64–67	DEAD	− (100)	+ (91)	− (100)
IGLJ	2	T	+ (100)	− (100)	− (100)
	4	G	+ (100)	+ (98)	− (100)
	7	T	+ (100)	+ (100)	− (100)
	10–12	EI(L)K TVL(A/T)	+ (98)− (100)	− (100) + (94)	− (100)− (100)
IGLC	14	S	+ (100)	+ (100)	− (100)
	17	Q E	+ (100)− (100)	− (100)+ (100)	− (100)− (100)
	20a	Gap	+ (100)	+ (100)	− (100)
	32	F	+ (100)	+ (100)	− (100)
	34	P	+ (100)	+ (98)	− (100)
	56	Gap	− (100)	+ (100)	+ (100)
	60	D/E K/R	+ (100)− (100)	− (100)+ (100)	− (100) + (100)
	65	T	+ (100)	− (100)	+ (100)
	91	H	+ (100)	+ (100)	− (100)
	102	F	+ (100)	− (100)	− (100)

+, −, presence and absence of amino acid residue(s) or gap in the IGL sequences. The number in parentheses is the percentage of occurrence of each marker in the functional IGL sequences. The two markers in IGLC sequences at positions 79 and 94–95, which distinguish IGL isotypes except frog, are not listed here (see Fig. 3).