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. 1999 Dec;10(12):4231–4246. doi: 10.1091/mbc.10.12.4231

Figure 4.

Figure 4

Radiation increases transcriptional activity of a full-length p21 promoter via MAPK signaling. A431-TR25-EGFR-AS cells and MDA-TR15-EGFR-CD533 cells were cultured and infected via a poly-l-lysine–conjugated adenovirus with constructs containing the full-length p21 promoter or a truncated p21 promoter. Twenty-four hours after infection, cells were pretreated with either PD98059 (10 μM) or control (DMSO), followed by irradiation (2 Gy). The ability of radiation to alter full-length and truncated p21 promoter activity by luciferase activity in either A431-TR25-EGFR-AS cells (A) or MDA-TR15-EGFR-CD533 cells (B) is shown. Data represent the means of multiple individual points from six separate experiments (±SEM); *p < 0.01 greater than control value; #p < 0.01 less than control value.