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Validation of Dra and CA IV expression by immunohistochemistry. Colonic samples were stained with antibodies against (a-d) Dra or (e-h) CA IV. Normal apical expression of proteins was observed in distal colon from uninoculated SW (a,e) and FVB (b,f) mice. By 9 dpi, partial loss of protein expression was observed in infected SW mice (c,g) compared with complete lack of expression in infected FVB mice (d,h). Original magnifications are 200×.
