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. 2008 Aug 7;10(4):R69. doi: 10.1186/bcr2131

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Copyright © 2008 Deng et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Circulating tumor cell (CTC) enrichment from blood of breast cancer patients using EpCAM and cytokeratin (CK) antibodies coupled to MACS microbeads. (a) CTCs enrichment strategy for three types of CTCs. This will be the mode to enrich all CTCs theoretically. (b) Image gallery showing CTCs recovered after enrichment with anti-CK antibody only; (c) Image gallery showing the higher number of CTCs recovered after enrichment with a combination of anti-CK and anti-EpCAM antibodies (the same blood sample as Figure 4(b)). (d) Plot showing CTC recovery rates after enrichment with anti-CK or a combination of anti-CK and anti-EpCAM in relationship to average cell anti-CK signal intensity.