Table 1.

Oligonucleotide primers employed at melting temperatures (T_M in °C) used to determine the expression of RLN2, human RXFP1 relaxin receptor (RXFP1), S100A4 and 18S transcripts in human breast cancer cell lines and xenograft tumours.

Primer	size	primer sequence (5'-3')	TM	cycles
S100A4	289 bp	sense – gaa ggc cct gga tgt gat ggt g	65°C	35
		antisense – cat ttc ttc ctg ggc tgc tta tc
RLN2	485 bp	sense – tct gtt tac tac tga acc aat tt	55°C	35
		antisense – cat ggc aac att tat tag cca a
RXFP1	243 bp	sense – ccc aat tct cta tac tct gac cac aag	65°C	35
		antisense – tca tga ata gga att gag tct cgt tga tt
18S	344 bp	sense – gtt ggt gga gcg att tgt ctg g	62°C	10
		antisense – agg gca ggg act taa tca acg c