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Alterations in tumor necrosis factor-alpha (TNF-α) gene expression in RAW 264.7 cells after culture with bacitracin (0.25, 1.25, and 3.75 mM) for 24 hours. The ratio of TNF-α and the housekeeping gene β-actin is calculated. Values (n = 4 to 5/group) are presented as mean ± standard error and are compared by one-way analysis of variance and Tukey's test: *P < 0.05 versus control; #P < 0.05 versus 0.25 mM bacitracin.
