Table 5.
Progressive cross-linking of involucrin using different Gln and Lys residues
| Cross-links | CEs from: | Cultured cells
|
Epidermisa | |
|---|---|---|---|---|
| 3 d | 6–7 d | |||
| Involucrin–involucrin | Gln-133-Lys-62 | 325 | 100 | |
| Gln-465-Lys-468 | 40 | 250 | 850 | |
| Gln-465-Lys-485 | 25 | 245 | 565 | |
| Gln-465-Lys-508 | 40 | 120 | ||
| Gln-489-Lys-468 | 195 | 415 | ||
| Gln-489-Lys-485 | 285 | 420 | ||
| Gln-489-Lys-508 | 90 | |||
| Gln-496-Lys-62 | 445 | 165 | ||
| Involucrin–envoplakin | Lys-378 | 35 | 120 | |
| Lys-468 | 150 | |||
| Lys-485 | 245 | 640 | ||
| Lys-508 | 85 | 35 | ||
| Involucrin–desmoplakin | Gln-456 | 140 | ||
| Gln-495 | 170 | |||
| Gln-496 | 20 | 1145 | 985 | |
| Involucrin–SPRs | Gln-308 | 30 | ||
| Gln-309 | 115 | |||
| Gln-368 | 50 | 65 | ||
| Gln-369 | 280 | 445 | ||
| Gln-426 | 85 | 85 | ||
| Gln-427 | 245 | 515 | ||
| Gln-455 | 65 | 70 | ||
| Gln-456 | 465 | 590 | 265 | |
| Involucrin–loricrin | Gln-308 | 35 | ||
| Gln-309 | 76 | |||
| Gln-369 | 165 | |||
| Gln-425 | 70 | |||
| Gln-426 | 115 | 260 | ||
| Gln-455 | 25 | |||
| Gln-456 | 57 | 80 | ||
Yield is in picomoles per milligram of CE protein.
a
Data are from saponified foreskin CEs of Steinert and Marekov (1997).