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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: Cancer Res. 2008 Oct 15;68(20):8456–8464. doi: 10.1158/0008-5472.CAN-08-2213

Figure 1. Generation and characterization of Id-specific T-cell clones.

Figure 1

A, Id-specific T-cell clones of different subsets were generated and selected based on Id-induced IFN-γ and IL-4 secretion using ELISA assay. B, flow cytometry analysis showing the expression of CD8+ or CD4+ T-cell surface marker and CD69 activation marker on three T-cell clones. C, cytokine secretion pattern of three T-cell clones. T-cell clones were stimulated with irradiated DC pulsed with Id protein or irrelevant mouse IgG2b protein. Cytokines in cell-culture media were quantified by ELISA. Representative results of three independent experiments are shown.