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The relationship between the amount of kidney total RNA template (62.5 to 500 ng) and RT-PCR products was evaluated (A). A linear relationship was found between the amount of total RNA template and the amount of PCR product in the range of 62.5 to 500 ng for renal angiotensinogen at 28 cycles of PCR (B). On the basis of these data, the amount of renal total RNA template was selected as 125 ng for the following analysis.
