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Western blot analysis of renal protein using the specific Ao polyclonal antibody. A, Representative autoradiograph of renal Ao Western blot from Ang II–infused and sham-operated rats on a H/S diet. B, Densitometric analysis of the immunoreactive bands showed that Ang II infusion significantly increased renal Ao protein levels by 88%. Similar observations were obtained from 2 other experiments. *P<0.05 vs H/S+Sham.
