Skip to main content
. Author manuscript; available in PMC: 2008 Oct 29.
Published in final edited form as: Hypertension. 2003 Jan;41(1):42–49. doi: 10.1161/01.hyp.0000050102.90932.cf

Figure 6.

Figure 6

Representative films of Western blot analysis of AngII-infused (A and B) or sham-operated (C and D) rats against rat angiotensinogen (A and C) and human angiotensinogen (B and D). A and C, The antibody against rat angiotensinogen does not recognize human angiotensinogen (the right blank lanes on panels A and C). Rat angiotensinogen was detected in plasma and urine before and after the administration of human angiotensinogen. B and D, Human angiotensinogen was detected only in the plasma collected from rats infused with human angiotensinogen. Human angiotensinogen was not detected in the urine in either AngII-infused or sham-operated rats. The lower bands at 50 kDa of rat plasma samples may be nonspecific binding. P, on the left, indicates 0.1 μL of plasma sample before the administration; U, 1.5% of urine sample collected every 30 minutes from 30 minutes before the purified human angiotensinogen infusion to 300 minutes after; P, on the right, 0.1 μL of plasma sample after the administration of human angiotensinogen; A, purified human angiotensinogen, 75 ng on Panel B, 25 ng on Panel D.