Figure 11.

Grd20-HA partially colocalizes with the TGN marker A-ALP. Strain SBY4-10A carrying plasmids pSB10 and pAH16 was fixed, spheroplasted, and costained with anti-HA and anti-ALP antibodies. The anti-HA antibody was detected with an alexa 488-conjugated secondary antibody, whereas the anti-ALP antibody was detected with a Texas Red-conjugated secondary antibody. The cells were viewed by epifluorescence using filters specific for each fluorochrome. Two fields stained for each antigen are shown, with the panels on the right representing images derived from a merge of the images for each antigen.