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. 1999 Dec;10(12):4263–4281. doi: 10.1091/mbc.10.12.4263

Figure 2.

Figure 2

A loss of Grd20p function causes a severe growth defect. The following strains were spotted onto YEPD plates from left to right: SNY17, SBY4-10A, SBY7-7A, SBY4-10A carrying pSB1, SBY4-10A carrying pSB1-125, and SBY4-10A carrying pSB1-1212 (see Table 2 for descriptions of plasmids). Approximately 5000, 500, and 50 viable cells were applied at the three positions (from top to bottom) for each strain. The plates were incubated at the indicated temperatures for 36 h before being photographed.