Figure 9.

Grd20p is necessary for normal actin polarization and organization. Strains SBY4-10A/pSB1 (GRD20) and SBY4-10A/pSB1-125 (grd20-1) were grown for several doublings at 22°C and then shifted to 36°C for 2 h, whereas strain SBY4-10A (grd20-Δ1) was propagated at 30°C before fixation. The cells were then fixed, stained with phalloidin conjugated to Texas Red, washed, mounted on slides, and visualized using differential interference contrast optics and by epifluorescence through a filter specific for Texas Red.