Table 2.
Plasmids used in this study
| Plasmid | Description | Reference or source |
|---|---|---|
| pSN55 | STE13-PHO8 gene fusion encoding A-ALP in pRS316 | Nothwehr et al., 1993 |
| pSN92 | PHO8 gene in pRS316 | Nothwehr et al., 1993 |
| pAH16 | STE13-PHO8 gene fusion encoding A-ALP in pRS314 | Nothwehr et al., 1999 |
| pSB1 | pRS316 containing the GRD20 gene | This study |
| pSB1-125 | pRS316 containing the grd20-1 temperature-sensitive allele | This study |
| pSB1-1212 | pRS316 containing the grd20-2 temperature-sensitive allele | This study |
| pSB1-139 | pRS316 containing the grd20-3 temperature-sensitive allele | This study |
| pSB1-23 | pRS316 containing the grd20-4 temperature-sensitive allele | This study |
| pSB1-65 | pRS316 containing the grd20-5 temperature-sensitive allele | This study |
| pSB10 | pRS316 containing the GRD20 gene with 3 copies of the HA epitope inserted at the C-terminus of the ORF | This study |
| pSB18 | pRS313 containing the grd20-1 temperature-sensitive allele | This study |
| pSB19 | pRS313 containing the SUC2 gene | This study |
| pSN179-A | pRS316 containing the KEX2 gene | This study |
| pSL2099 | pRS315 containing a GAL1-STE3∷myc fusion construct | Davis et al., 1993 |