Table 3.

Relationship between various phenotypes in grd20 temperature-sensitive strains

Allele	Growth rate at 22°Ca	Growth rate at 36°C	% CPY secretion at 36°Cb	% intracellular CPY in pro formb	% cells with actin cablesc
GRD20	++++	++++	<10, <10	<5, <5	81
grd20-1	+++	−	47, 46	78, 64	0
grd20-2	+++	++	45, 20	43, 10	59
grd20-3	+++	−	34	70	0
grd20-4	+++	−	36	73	0
grd20-5	+++	++	32	29	43

The strains listed from the top to bottom correspond to SBY4-10A transformed with the following plasmids, respectively: pSB1, pSB1-125, pSB1-1212, pSB1-139, pSB1-23, and pSB1-65. 

^aFor determination of the growth rate, cells were spotted onto YEPD plates and incubated at the indicated temperatures as described in the legend to Figure 2. The relative rates of growth were qualitatively scored after 48 hr of growth with ++++ indicating maximal growth and − indicating little or no growth detected (i.e., refer to the data for the grd20-1 strain at 36°C). 

^bThe percent CPY secretion was determined as described in the legend to Figure 3B. The percent intracellular CPY in the nonvacuolar processed pro form was determined by dividing the amount of pro-CPY in the intracellular fraction by the total CPY in the intracellular fraction. The two percent secretion and percent pro-CPY values given for GRD20, grd20-1, and grd20-2 are the result of two independent determinations, whereas the result of a single experiment is given for the other strains. 

^cThe yeast strains were grown and stained with Texas Red-conjugated phalloidin according to the legend for Figure 9. A total of 100–150 cells from each strain were then scored for the presence of actin cables (see MATERIALS AND METHODS). The analysis was limited to cells with small buds and a single nucleus, because in wild-type cells actin cables in this phase of the cell cycle were easier to identify than at other phases of the cell cycle.