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. Author manuscript; available in PMC: 2009 Oct 10.
Published in final edited form as: J Mol Biol. 2008 Jul 25;382(3):638–651. doi: 10.1016/j.jmb.2008.07.045

Fig. 7. Trak1 overexpression inhibits trafficking of EGF-EGFR complexes from early endosomes to the lysosomal pathway.

Fig. 7

(a) HeLa cells expressing GFP or GFP-tagged Trak1, Trak1Δhyrt, Trak1Δ1,Trak1Δ2, and Hrs were allowed to internalize TR-EGF for 10 minutes and then chased for 1 hour at 37°C. Cells were stained with a primary antibody against EEA1 and a CY5-conjugated secondary antibody. Arrowheads and asterisks indicate transfected and untransfected cells, respectively. Bar,10 µm. (b) HeLa cells were transiently transfected with GFP vector, GFP-tagged Hrs (Hrs) or the following GFP-tagged Trak1 constructs: Trak1 (WT), Trak1Δhyrt (Δh), Trak1Δ1 (Δ1), or Trak1Δ2 (Δ2). Untransfected HeLa cells were used as a control (CTL). The amount of remaining TR-EGF after 1-hour chase was quantified and expressed as a percentage of the initially internalized TR-EGF. Data represent mean ± s.e.m. from three independent experiments. The asterisks indicate a statistically significant difference (p < 0.05) from the GFP vector-transfected cells, whereas the pound sign indicates a statistically significant difference (p < 0.05) from the GFP-Trak1 WT-transfected cells.