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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: J Immunol. 2008 Oct 15;181(8):5791–5802. doi: 10.4049/jimmunol.181.8.5791

Figure 1. Accumulation of Gr-1+CD11b+ cells in spleens of tumor-bearing mice.

Figure 1

Splenocytes from naïve or tumor-bearing mice (3 weeks after tumor inoculation, tumor size 1.5 cm in diameter) were stained with anti-CD11b and anti-Gr-1 antibodies. A. Typical example of flow cytometry analysis. B. The percentage of Gr-1+CD11b+ cells in spleen from naïve or tumor-bearing mice on the C57BL/6, BALB/c, or FVB/N backgrounds as indicated. Each group included from 3 to 8 mice. Mean and standard deviation are shown. Differences between proportion of Gr-1+CD11b+ cells in spleens from naïve and tumor-bearing mice were statistically significant for all tumor models (p<0.05). C. Bone marrow cells from naïve mice were cultured for 5 d with 10 ng/ml GM-CSF and IL-4 in the presence of control (3T3) or conditioned media from indicated tumor cell lines. Cells were collected, labeled with anti-Gr-1 and anti-CD11b Abs, and analyzed by flow cytometry. * - statistically significant differences from control (3T3) level (p<0.05).