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. Author manuscript; available in PMC: 2009 Sep 24.
Published in final edited form as: Mol Cell Endocrinol. 2008 May 21;292(1-2):79–86. doi: 10.1016/j.mce.2008.05.007

Figure 1.

Figure 1

Strategy for subdividing the RING-domain of RUSH/HLTF. The RING-finger plus 2 amino acids at each end equals a 48 amino acid region (amino acids 755–802) that was divided into four non-overlapping peptides of 12 amino acids (36 nucleotides) each. Three additional double-stranded oligonucleotides were designed to encode peptides that span the ends of the original four peptides in case the boundaries of those peptides interrupted the sequence(s) required for binding. Sufficient spacer nucleotides were introduced at the 5′-end of each RING-fragment to ensure that the desired reading frame was preserved.