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. Author manuscript; available in PMC: 2009 Sep 24.
Published in final edited form as: Mol Cell Endocrinol. 2008 May 21;292(1-2):79–86. doi: 10.1016/j.mce.2008.05.007

Figure 4.

Figure 4

Western analysis confirms enhanced binding of KVIRLDKIS to MBP-RING1. Left lane, molecular size marker (60 kDa). Binding of MBP-RING1 to RFBP1 (second lane) and RFBP8 (KVIRLIKIS, third lane) is comparable. Binding of MBP-RING1 to RFBP8m (KVIRLDKIS, fourth lane) is increased 2–4-fold. Negligible binding of MBP-RING1 to RFBP5 (fifth lane) is provided as a negative result (please see Figure 2). All binding comparisons were determined by computer-assisted image analysis as described.