Table 3.
Number | Sequence | Restriction sitea |
---|---|---|
256 | 5′-ACGCTCGCGACTAACAGCACAAGAGCGGAAAGATG-3′ | NruI |
257 | 5′-CGGGATCCGTCGACCAGATAAAATATTTCTAGAACACCTCC-3′ | BamHI |
660 | 5′-GCTCTAGATCGAGCCGGTCAACGGAAAT-3′ | XbaI |
661 | 5′-CTCCAAATATAAAACGCGGATGAACGGTCATTATGGTGGTGATTT-3′ | – |
662 | 5′-AAATCACCACCATAATGACCGTTCATCCGCGTTTTATATTTGGAG-3′ | – |
663 | 5′-GGGGATCCTTGCTACTTTGGATGGTGGTG-3′ | BamHI |
664 | 5′-CGCGGATCCAGGAGGAAAAATATGAAATCTAAAAATAATTATTTCAAAC-3′ | BamHI |
665 | 5′-AAGTCGACTTATGCTTTTTTTCTCCAAATATAAAA-3′ | – |
the indicated restriction endonuclease site is underlined within each oligonucleotide sequence.