Table I. RT-qPCR primers.
| Primer | Accession no. | Sequence | Tm | Amplicon |
|---|---|---|---|---|
| °C | bp | |||
| MmAxin2Exon3For | NM_027732 | GACGCACTGACCGACGATTCCA | 61.5 | 120 |
| MmAxin2Exon4Rev | ATTGGCCTTCACACTGCGATGC | 60.7 | ||
| MmGAPDHExon3For | NM_008084 | GCAATGCATCCTGCACCACCA | 61.1 | 138 |
| MmGAPDHExon4Rev | TTCCAGAGGGGCCATCCACA | 61.1 | ||
| MmHNF1aExon5For | NM_009327 | CGCGTGGCGAAGATGGTCAAGT | 62.3 | 124 |
| MmHNF1aExon6Rev | TCATGGGTGTGCCCTTGTTGAGG | 61.8 | ||
| MmLef1Exon3For | NM_010703 | TTCTCCACCCATCCCGAGGACA | 62.1 | 122 |
| MmLef1Exon4Rev | ACGGGTGGGATCCCGGAGAAAA | 62.8 | ||
| MmMycExon2For | NM_010849 | CACCACCAGCAGCGACTCTGAA | 61.7 | 125 |
| MmMycExon3Rev | TGTGGCCTCGGGATGGAGATGA | 62.4 | ||
| MmPpp2r5dExon11For | NM_009358 | CGCATCCTCCCCATCATGTTTCC | 60.2 | 135 |
| MmPpp2r5dExon12Rev | TGTGCAGTCGTCAAACAGCTTCTGG | 61.5 | ||
| MmRhoUExon2For | NM_133955 | TGTAGATGGGCGGCCTGTGAGA | 62.5 | 119 |
| MmRhoUExon3Rev | CCACGCTGAAGCACAGCAGGAA | 62.4 | ||
| MmTcf4Exon7For | NM_013685 | GGGCAATCCAGGAACCCTTTCG | 60.6 | 150 |
| MmTcf4Exon8Rev | GAAGACGGCAAACCCGGAGGAA | 61.8 | ||
| MmWnt7aExon3For | NM_009527 | GATGCCCGGGAGATCAAGCAGA | 61.3 | 122 |
| MmWnt7aExon4Rev | GAGCCTGACACACCATGGCACT | 61.9 | ||
| MmWnt10aExon1For | NM_009518 | TTCTGGGCGCTCCTGTTCTTCC | 61.7 | 123 |
| MmWnt10aExon2Rev | CAGGCACACTGTGTTGGCGTTG | 61.6 |
These sequence data are available from GenBank/EMBL/DDBJ under the accession numbers listed. Tm, melting temperature.