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. 2008 Nov 3;183(3):441–455. doi: 10.1083/jcb.200807043

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© 2008 Buchan et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — Stress granule assembly mutants are not deficient in their ability to both translationally repress and stabilize mRNA during stress. (A) Log-phase BY4741 wild-type and isogenic knockout strains were washed and incubated in media +/− glucose at 30°C for 10 min, followed by ³⁵S-met/cys labeling for 5 min. Lysates were prepared and separated by SDS-PAGE for PhosphorImager analysis. (B) Log-phase BY4741 WT and isogenic knockout strains transformed with pRP1192 were resuspended in media +/− glucose, followed by doxycycline addition to transcriptionally repress the MFA2-pG mRNA reporter. Thus, only decay of existing MFA2-pG mRNA was examined. Time points were taken and analyzed via Northern blot. mRNA half-lives (right) are indicated.