Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Nov 3;183(3):441–455. doi: 10.1083/jcb.200807043

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 Buchan et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — Yeast temporal analysis reveals accumulation of Pab1 in P bodies before formation of P body–distinct stress granules. (A) Log-phase yRP840 strain, transformed with pRP1660, was glucose deprived for 5 min and followed over time. For technical reasons, identical cells at the 0-min time point are not shown; instead a typical image of this time point is shown. Orange arrowheads indicate initial accumulation of Pab1-GFP in preexisting P bodies. Purple arrowheads indicate appearance of P body–distinct stress granules. Images are typical of multiple independent experiments. Images were taken from Video 1 (available at http://www.jcb.org/cgi/content/full/jcb.200807043/DC1). (B) Zoom panels from the 19-min time point (white boxes). Arrowheads indicate common occurrence of faint P-body foci colocalizing with stress granules. Purple arrowhead indicates same foci in A, turquoise arrowheads indicate location of additional faint P bodies. Right: Dcp2-mCh image at 10x normal scaling intensity, indicating relative difference in Dcp2-mCh abundance between P bodies and stress granules. Asterisk indicates auto-fluorescent vacuolar signal.