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. 2008 Nov 3;183(3):513–526. doi: 10.1083/jcb.200804048

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Figure 2. — Immunofluorescence microscopy showing localization of Vps26 to Rab7- and Rab5-positive endosomes. HeLa cells were transfected with plasmids encoding GFP-Rab7 (A–C) or GFP-Rab5a (D–F), fixed, permeabilized, and immunostained with rabbit polyclonal antibody to Vps26 followed by Alexa Fluor 594–conjugated donkey anti–rabbit IgG. Cells were examined by confocal microscopy. (A and D) GFP fluorescence, green channel. (B and E) Alexa Fluor 594 fluorescence, red channel. (C and F) Merged images; yellow indicates colocalization. Arrows indicate examples of foci where proteins colocalize. Bar, 10 μm.