Figure 4.
Immunoelectron microscopy localization of GFP-Rab7 and Vps26. Ultrathin cryosections of HeLa cells expressing wild-type GFP-Rab7 were immunogold labeled with antibodies to GFP (10-nm gold particles) and Vps26 (15-nm gold particles). Arrows indicate the presence of GFP-Rab7 on buds adjacent to the vacuolar part of endosomes (A) and on tubules (B). Tubules and buds are also labeled for Vps26. Bars, 200 nm.