Figure 6.
Inhibitors of SAHH interfere with methylation and elongation of poly(A)+ RNA. XTC cells were simultaneously pulse labeled with l-[methyl-3H]methionine and [U-14C]uridine in the presence or absence of 2-deoxyadenosine (2dAde), tubercidine, or DRB, and the incorporation of radioactive precursors in poly(A)+ RNA was analyzed as detailed in MATERIALS AND METHODS. The results of two independent experiments are combined and shown with error bars. Incorporation of [U-14C]uridine in poly(A)+ RNA of control cells was between 290 and 373 cpm, and incorporation of [3H]methyl was between 30 and 74 cpm.