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. 1999 Dec;10(12):4283–4298. doi: 10.1091/mbc.10.12.4283

Figure 7.

Figure 7

Nuclear translocation of full-length and mutated xSAHH in transfected cells. (A) Endogenous nucleoplasmic xSAHH stained with mAb 32-5B6 in untransfected A6 cells. A mitotic cell is marked by a long arrow. Full-length (B and C) and C-terminally (D-F) and N-terminally deleted (G and H) MT-xSAHH was detected in Xenopus A6 cells using mAb 9E10 after transient transfection as detailed in MATERIALS AND METHODS. Cells were fixed and processed for immunofluorescence 120 h (B) or 40–48 h (C–H) after transfection. Cells were transfected with pCS2+MT containing cDNA encoding full-length xSAHH (B and C), xSAHHΔ425–433 (D), xSAHHΔ402–433 (E), xSAHHΔ361–433 (F), xSAHHΔ1–13 (G), or xSAHHΔ1–21(H). Transfected cells with predominantly nuclear localization of MT-xSAHH are marked with arrowheads, and cells with predominantly cytoplasmic localization are marked with short arrows. Bar, 50 μm.