Figure 8.

Influence of N-terminal and C-terminal deletions on intracellular localization of MT-xSAHH in transfected A6 cells. (A) Full-length MT-xSAHH or the truncated forms explained in B were expressed in A6 cells after transient transfection. The percentages of transfected cells showing localization of the MT-xSAHH exclusively (N) or predominantly in the nucleus (N>C), in the cytoplasm (C>N), or at similar levels in both compartments (N=C) are compiled from two or three independent experiments and plotted. Several hundred cells were analyzed for each individual fusion protein, except for xSAHHΔ361–433, where n was 165, because this product was either toxic or unstable, resulting in a low percentage of evaluable cells. (B) The N terminus and the C terminus of the fusion proteins encoded by the cDNAs used for transfection are shown, with numbers referring to the wt-xSAHH2 aa sequence. Only the last of six N-terminal myc tags are shown in italics. The original xSAHH sequence is shown in bold letters; linker sequence is shown in plain uppercase letters; and aa exchanged by mutagenesis are underlined.