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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Cancer Res. 2008 Aug 1;68(15):6341–6349. doi: 10.1158/0008-5472.CAN-07-5769

Figure 1.

Figure 1

Effect of aging on DC:T-cell interaction. (A) CD8+ T cells from OT-I transgenic mice were stimulated for 3 days with irradiated class I OVA-peptide(257−264) - pulsed young and old DCs at the R/S ratios indicated. Proliferation was determined as described in Materials and Methods. Data are representative of 4 independent experiments with at least 2 mice per age group. (B) Young and old DCs were incubated with FITC-OVA protein for the indicated times and phagocytosis analyzed by FACS. Specific uptake was assessed by subtracting the MFI of cells incubating at 4°C from the value at 37°C. (C) DCs were pulsed for 6 hrs with FITC-labeled OVA peptide(257−264) and then stained with PE-conjugated MHC class I. Dot plots of one representative experiment per age group of 3 performed are shown. (D) Phenotype of young and old OVA PP-DCs. DCs were pulsed for 6 hrs with OVA peptide(257−264) and stained with indicated antibodies. The percentages of positive cells over isotype-matched controls are shown. Results represent the means ± standard error of the mean (n= 10 mice per age group) of three independent experiments. * p<0.0025; ** p< 0.05; ***p < 0.01.