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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Cancer Res. 2008 Aug 1;68(15):6341–6349. doi: 10.1158/0008-5472.CAN-07-5769

Figure 6.

Figure 6

FACS analysis of immune cell subsets infiltrating B16-OVA tumors. Tumors were harvested seven days after DPBS, young or old OVA PP-DC injection and used to prepare a single cell suspension for FACS analysis. The live population was gated on, and the percentage of cells co-expressing CD45 and indicated cell surface molecules determined. (A) Pie charts representing the contribution of each subset tested relative to the immune infiltrate in one tumor-bearing mice immunized with DPBS, 2 × 106 (1×) young OVA PP-DCs, 2 × 106 (1×) and 4 × 106 (2×) old PP-DCs. (B) The average total number of each subset was determined and represented as mean % double positive cell ± standard error of the mean of 6 independent experiments. *p < 0.01, ** p < 0.005: young PP-DCs vs old PP-DCs; ***p < 0.02 young and old PP-DCs vs DPBS.