Fig. 2.
Conductance and voltage-gating properties of wild-type, T8M, and N206S channels. A: comparison of macroscopic junctional conductance of Cx26 wild-type (n = 15), T8M (n = 16), and N206S channels (n = 20) in transiently transfected HeLa cells showed that they were not statistically different from each other (ANOVA, P < 0.05). Data are means ± SE. B: junctional currents from HeLa cell pairs transfected with wild-type Cx26, T8M, and N206S were recorded during application of a series of transjunctional voltages (Vjs) ranging from +110 to −110 mV in 20-mV increments (left). The normalized junctional conductance (means ± SE) versus Vjs was plotted (right). Cx26 wild-type and mutant T8M and N206S channels demonstrated very little voltage dependence.