Fig. 3.
Single channel properties of wild-type, T8M, and N206S channels. Single channel currents recorded from cell pairs expressing wild-type, T8M, and N206S at a Vj of ±110 mV in transiently transfected Neuro-2A cells. A: current histograms of the single channels for wild-type and mutant connexins revealed unitary conductances between 98 and 123 pS in 120 mM K+-aspartate− (left). Solid line represents the zero junctional current and the dashed lines indicate open-state currents. The averaged unitary conductances of wild-type, T8M, and N206S channels were statistically indistinguishable from each other (ANOVA, P < 0.05) (right). B: with the use of 120 mM TEA+-aspartate− pipette solution (left), Cx26 wild-type, T8M, and N206S channels had unitary conductances between 58 and 63 pS and the mean unitary conductances for all three channels were comparable to each other (ANOVA, P < 0.05, right). Data represented as means ± SE.