Fig. 7.

Relative intensity of recipient cells for LY and EtBr as a function of junctional conductance (g_j). The relative intensities of recipient cells of Cx26 wild-type, T8M, and N206S junctions in different cell pairs were analyzed at the 12-min time point and plotted against the g_j. A: relationship between the relative intensity and the g_j for wild-type, T8M, and N206S for LY was linear [R² values were 0.90 (straight line), 0.96 (dashed line) and 0.92 (dotted line), respectively]. LY passage between pairs of cells expressing wild-type, T8M, and N206S was comparable to each other, and statistically were not different (ANOVA, P < 0.05). B: EtBr transfer between cell pairs expressing wild-type Cx26 showed a modest linear correlation between relative intensity of recipient cell and g_j with a R² value of 0.92 (straight line). T8M and N206S channels showed only a weak correlation between g_j versus relative intensity of recipient cell and greatly reduced EtBr permeability. The slopes of fitted lines for T8M (dashed fitting line) and N206S (dotted fitting line) channels were 27.6% and 28% of that of wild-type Cx26.