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. 2008 Jul 30;295(4):C923–C930. doi: 10.1152/ajpcell.00237.2008

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Fig. 5. — Detection of V-ATPase subunits and olfactory markers in adult mouse olfactory homogenate by immunoblotting. Twenty-five micrograms of B1^+/− mouse OE (lane 1) and 50 μg of B1^+/+ mouse OE (lane 2) were subjected to SDS-PAGE and blotted with antibodies against V-ATPase A and B1 subunits, olfactory marker protein (OMP), and protein gene product 9.5 (PGP 9.5). Total kidney homogenate from B1^+/+ (lane 3) and B1^−/− (lane 4) mouse was used as control. V-ATPase A subunit was detected in all samples, and B1 was detected in olfactory mucosa and wild-type kidney samples. Immunoblotting of OE samples with antibody against the B1 isoform as whole serum yielded 2 bands: one at 56 kDa and another (less intense) lower-molecular-weight band. Preincubation of the antibody with the immunizing peptide completely abolished the specific 56-kDa band in all lanes in which it had been detected. Both olfactory markers were expressed in OE, but not in kidney samples.