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IGF effects on Foxo4. Myotubes were pretreated for 30 min with 10 ng/ml IGF followed by 2 h 6 ng/ml TNF. A: as measured by Western blot, pretreatment with IGF elevates phospho-Foxo4 (*P < 0.01, n = 3), and TNF does not reverse IGF-induced phosphorylation. B: nuclear extracts were prepared from myotubes treated as in A. TNF induced Foxo4 nuclear translocation (*P < 0.05, n = 3) that was not prevented by pretreatment with IGF.
