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. 2008 Nov 3;118(12):3904–3916. doi: 10.1172/JCI36168

Figure 5. VEGF-induced EC migration and tube formation were inhibited by AIP1 overexpression but augmented by AIP1 deletion.

Figure 5

(A–D) AIP1 overexpression inhibits EC migration. HUVECs were infected with adenovirus expressing WT (Ad-AIP1) in the presence of 4 μm doxycycline for 24 hours. (A) AIP1 expression was determined by Western blot with anti-AIP1. (B) Cells were cultured in serum-free media for overnight and subjected to a transwell migration assay and (C with quantification in D) monolayer “wound injury” assay in response to VEGF (10 ng/ml). (E and F) AIP1 overexpression inhibits EC tube formation. HUVECs were infected with Ad-AIP1 and subjected to a Matrigel tube formation assay VEGF (10 ng/ml) (E with quantification in F). (G–J) AIP1 deficiency augmented on EC migration and tube formation. WT and KO mouse lung microvessel ECs (MLECs) were cultured in 0.5% FBS overnight and subjected to EC migration and tube formation as described above. (G) AIP1 expression was determined by Western blot with anti-AIP1. Quantification for EC migration in transwell assay (H), “wound injury” assay (I), and tube formation assay (J) are shown. Data presented are mean ± SEM of the triplicates from 3 independent experiments. *P < 0.05.