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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Biol Reprod. 2008 May 7;79(2):337–347. doi: 10.1095/biolreprod.108.069328

FIG. 2.

FIG. 2

Identification of PADI6 by microsequencing. The 75-kDa band was excised from a gel containing egg proteins, and peptides were analyzed by LC-MS. Microsequenced peptides covered 18% of the mouse PADI6 sequence shown (UniProt primary accession no. Q8K3V4) with 100% identity (underlined portions). Bold and dashed underline portions indicate putative YWHA binding domains in PADI6 as indicated by a YWHA motif search in Scansite [34]. Both of the regions contain the ArgXXSer sequence associated with YWHA binding. In these sequences, Ser-219 and Ser-400 (asterisks) also are highly probable serine phosphorylation sites (NetPhos 2.0 Server [35]). Ser-219 and Ser-400 are putative targets of PKA and RPS6KA1, and Ser-400 also may be a PKC site as well as a AKT1 site (Scansite and NetPhosK 1.0 Server [34, 35]).