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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Biol Reprod. 2008 May 7;79(2):337–347. doi: 10.1095/biolreprod.108.069328

FIG. 3.

FIG. 3

Immunoprecipitation (IP) of HSPA8 protein followed by SDS-PAGE and immunoblotting indicates that the p(S)YWHA binding motif antibody does not recognize HSPA8. Left) HSPA8 IP. Right) Control IP. The HSPA8 protein IP pellet sample was divided equally by volume, resolved by SDS-PAGE, blotted, and probed with the anti-p(S)YWHA binding motif antibody (lane 1) or anti-HSPA8 (lane 3). The HSPA8 IP pellet sample is not labeled by the anti-p(S)YWHA binding motif antibody, although the protein labeled by this antibody is in the flow-through fraction (lane 2). Control IP used rabbit serum in place of HSPA8 antibody. The HSPA8 protein was not immunoprecipitated (lane 4) though the protein was present in the flow-through protein faction (lane 5).