FIG. 4.

Western blots of egg and oocyte extracts after two-dimensional polyacrylamide gel electrophoresis provide additional evidence that anti-p(S)YWHA binding motif antibody labels PADI6 in eggs. For each blot shown, an equal number of cells were lysed, and the proteins were electrophoresed in two dimensions, blotted, and probed with the indicated antibody. Immunoreactivity for anti-PADI6 antibody was observed at approximately 75 kDa and an isoelectric point (pI) of approximately 5.7 in egg and oocyte blots (B and D), whereas anti-p(S)YWHA binding motif antibody reactivity was seen in a similar location only in eggs (A and C). The two-dimensional blots are representative of three experiments.