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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Biol Reprod. 2008 May 7;79(2):337–347. doi: 10.1095/biolreprod.108.069328

FIG. 6.

FIG. 6

Western blots of GST-YWHA pull-down assay of egg extracts treated with lambda phosphatase. A) GST-YWHA bound and unbound fractions probed with anti-p(S)YWHA demonstrate that the phosphatase treatment removes phosphates in these extracts, because the antibody no longer recognizes any protein. The control lane consists of an extract of eggs not treated with lambda phosphatase and not used in the pull-down assay, and it confirms that the same antibody preparation is effective. B) The same blot as in A reprobed with anti-PADI6. The PADI6 protein was present in the GST-YWHA unbound fraction and in the untreated control but was undetectable in the GST-YWHA bound lane, providing additional evidence that the YWHA/PADI6 interaction is phospho-dependent. This experiment was repeated three times with identical results.