Table 1.

	MCF7	SKBR3	DLD-1
AdWt	8	0.63	1.33
AdIP2	>100	>100	4.77

DLD-1 colon cancer cells carrying an APC mutation that results in constitutively high β-catenin signalling and cell lines in which the Wnt signalling pathway is not activated in normal culture conditions (MCF7, SKBR3) were infected with AdIP2 and a wild-type adenovirus type 5 as positive control at different multiplicity of infection. Six days later, the number of viable cells was determined using a MTT assay. Dose–response curves were generated and concentrations killing 50% of cells (EC50-values) were determined using untreated cells as reference and are presented in pfu.